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Michael Massiah
Oklahoma State University
Assistant Professor, Department of Biochemistry & Molecular Biology
Address: 246 Noble Research Center, OSU, Stillwater, OK 74078
Phone: 405-744-3797 Fax: 405-744-7799
Email: massiah@biochem.okstate.edu
URL: http://biochem4.okstate.edu/~massiah
Ph.D., Chemistry and Biochemistry, University of Maryland, 1996 Lab Photo
Postdoctoral:

Dept. of Biophysics, Memorial Sloan-Kettering Cancer Institute, 1996-97
Research Emphasis:
Related Activities:
 
Research Description:

Our Research aim is to use high resolution multi-dimensional Nuclear Magnetic Resonance (NMR) spectroscopy to study Midline-1 (Mid1), a Ring Finger (RF) protein associated with Opitz Syndrome (OS). Patients with mutations in Mid1 have dramatic structural abnormalities along their body's midline that may be lethal or require many surgeries for survival. Mutations in Mid1 disrupt its interaction with microtubules, and inhibit its ability, as an ubiquitin E3 ligase, to regulate protein phosphatase 2A (PP2A), a critical enzyme required for G1/S transition, S phase progression, and cell division. The functions of Mid1 are also believed to be affected in other human genetic syndromes since many of these syndromes share characteristic phenotypes as OS and are found to have defects in the mid1 gene. Other proteins containing Ring Finger domains are found to be associated with human diseases such as breast cancer, Parkinsons disease, microphthalmia with linear skin defects, acute promyelocytic leukemia, and astrocytoma.
 
Recent Publications:

Massiah, MA, Saraswat S, Azurmendi HF, Mildvan AS (2003) Solution Structure, Mutagenesis, and NH Exchange Studies of the MutT-Mg2+-8-oxo-dGMP complex. J Molecular Structure, submitted.

Saraswat V, Massiah MA, Lopez G, Amzel LM, Mildvan AS (2002) Interactions of the products, 8-Oxo-dGMP, dGMP, and Pyrophosphate with the MutT Nucleoside Triphosphate Pyrophosphohydrolase. Biochemistry, Web Release 12-12-02.

Legler PM, Massiah MA, Mildvan AS (2002) Mutational, Kinetics, and NMR Studies of the Mechanism of E.Coli GDP-Mannose Mannosyl Hydrolase, an Unusual Nudix Enzyme. Biochemistry, 41 (35), 10834-10848.
 
Selected Additional Publications:

 Marks GT, Harris TK, Massiah MA, Mildvan AS, Harrison DHT (2001) Mechanistic Implications of Methylglyoxal Synthase Complexed with Phosphoglycolohydroxamic Acid as Observed by X-ray crystallography and NMR spectrocopy. Biochemistry, 40 (23), 6805-6818.

Dinkova-Kostova AT, Massiah MA, Bozak RE, Hicks RJ, Talalay P (2001) Potency of Michael reaction acceptors as inducers of enzymes that protect against carcinogenesis depends of their reactivity with sufhydryl groups. Proc Natl Acad Sci, 98 (6), 3404-3409.

Czerwinski RM, Harris TK, Massiah MA*, Mildvan AS, Whitman CP (2001) The Structural Basis for the Perturbed pKa of the Catalytic Base in 4-Oxalocrotonate Tautomerase: Kinetic and Structural Effects of Mutations of Phe-50. Biochemistry, 40(7), 1984-1995.

Massiah MA, Viragh C, Reddy PM, Kovach IV, Johnson J, Rosenberry TL, Mildvan AS (2001) Short, Strong Hydrogen bond at the Active Site of Human Acetyl Cholinesterase: Proton NMR Studies. Biochemistry, 40 (19), 5682-5690.